F.Boliver and R. Rodrigeuz constructed first artificial vector plasmid was

Options

A. pUC19
B. pUC 101
C. pBR 322
D. Ti plasmid

Answer

**Step-by-Step Solution:**

1. **Identify the Scientists**: The question mentions two scientists, F. Boliver and R. Rodriguez, who constructed the first artificial vector plasmid.

2. **Understand the Options**: The options provided are:
- PUC19
- PUC101
- PBR322
- TI Plasmid

3. **Recall the Contribution**: It is known that F. Boliver and R. Rodriguez are credited with constructing the first artificial vector plasmid.

4. **Identify the Correct Plasmid**: The first artificial vector plasmid constructed by F. Boliver and R. Rodriguez is **PBR322**. The name PBR322 can be broken down as follows:
- "P" stands for plasmid.
- "BR" stands for Boliver and Rodriguez.
- "322" is a sequence number.

5. **Additional Information**: PBR322 is 4361 base pairs long and contains two antibiotic resistance genes, which are important for its function as a cloning vector.

6. **Eliminate Other Options**:
- **PUC19** and **PUC101**: These plasmids were also developed by the same scientists but are not the first artificial vector plasmid.
- **TI Plasmid**: This is a tumor-inducing plasmid found in Agrobacterium and is not related to the work of Boliver and Rodriguez.

7. **Conclusion**: Therefore, the answer to the question is **PBR322**.

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