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The process by extraction of the separat...

The process by extraction of the separated bands of DNA from agarose gel is known as

A

Spooling

B

Down streaming

C

Elution

D

Emasculation

Text Solution

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The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding the Context**: The question refers to the extraction of separated bands of DNA from agarose gel. This process is commonly used in molecular biology to analyze DNA fragments. 2. **Electrophoresis Process**: Initially, DNA samples are loaded into an agarose gel. The gel acts as a medium through which the DNA can be separated based on size. 3. **Movement of DNA**: DNA is negatively charged due to its phosphate backbone. When an electric current is applied, the DNA fragments migrate towards the positive electrode. 4. **Separation of DNA Bands**: As the DNA moves through the gel, smaller fragments travel faster and further than larger ones, resulting in the separation of the DNA into distinct bands. 5. **Staining the DNA**: To visualize the separated DNA bands, the gel is stained with a dye such as ethidium bromide. This dye intercalates with the DNA and fluoresces under UV light. 6. **Exposure to UV Light**: After staining, the gel is exposed to UV radiation, which allows the separated bands of DNA to be seen. 7. **Extraction of DNA Bands**: The final step involves cutting out the desired bands of DNA from the agarose gel. This process of extracting the DNA from the gel is known as **elution**. 8. **Conclusion**: Therefore, the process of extracting the separated bands of DNA from agarose gel is called **elution**. **Final Answer**: The process by which extraction of the separated bands of DNA from agarose gel is known as **elution**. ---
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