In staggered cutting the fragment of DNA contain

Options

A. Sticky, identical ends
B. Non sticky identical ends
C. Sticky complimentary ends
D. Non sticky, Non complimentary ends

Answer

To solve the question about staggered cutting of DNA fragments, we need to understand the concept of DNA cutting, particularly by restriction enzymes. Here’s a step-by-step breakdown of the solution:

### Step-by-Step Solution:

1. **Understanding Staggered Cutting**:
- Staggered cutting refers to the way restriction enzymes cut DNA at specific sequences. Instead of cutting straight across both strands, they cut at slightly different positions on each strand, leading to overhangs.

2. **Formation of Sticky Ends**:
- When DNA is cut in a staggered manner, it results in the formation of what are known as "sticky ends." These are short single-stranded overhangs that can easily anneal with complementary sequences of DNA.

3. **Identifying the Options**:
- The question provides four options:
- A) Sticky identical ends
- B) Non-sticky identical ends
- C) Sticky complementary ends
- D) Non-sticky complete non-complementary ends

4. **Analyzing the Options**:
- **Option A**: Sticky identical ends - This is incorrect because staggered cutting does not produce identical ends.
- **Option B**: Non-sticky identical ends - This is also incorrect for the same reason as option A.
- **Option C**: Sticky complementary ends - This is correct because staggered cuts produce sticky ends that can pair with complementary sequences.
- **Option D**: Non-sticky complete non-complementary ends - This is incorrect as staggered cutting does not produce non-sticky ends.

5. **Conclusion**:
- The correct answer is **Option C: Sticky complementary ends**. This is because staggered cuts create overhangs that are sticky and can bind to complementary sequences.