An analysis of chromosomal DNA using the Southern hybridization technique does not use:

Options

A. PCR
B. Electrophoresis
C. Blotting
D. Autoradiography

Answer

To solve the question regarding the Southern hybridization technique and what it does not use, we can break down the process step by step.

### Step-by-Step Solution:

1. **Understanding Southern Hybridization**:
- Southern hybridization is a technique used to detect specific DNA sequences in DNA samples. It involves the transfer of DNA from an agarose gel to a membrane and subsequent hybridization with a labeled probe.

2. **Identifying the Components of the Technique**:
- The Southern hybridization technique typically involves several key steps:
- **Electrophoresis**: This is used to separate DNA fragments based on size. DNA samples are loaded into a gel and subjected to an electric field, allowing smaller fragments to move faster than larger ones.
- **Blotting**: After electrophoresis, the DNA is transferred from the gel to a membrane (blotting) where it can be probed.
- **Autoradiography**: This step involves using a labeled probe that binds to the specific DNA sequence of interest. The binding can be visualized using autoradiography, which reveals the location of the probe on the membrane.

3. **Analyzing the Options**:
- **Option A: PCR (Polymerase Chain Reaction)**: This technique is used to amplify specific DNA sequences but is not a part of the Southern hybridization process itself.
- **Option B: Electrophoresis**: This is indeed used in Southern hybridization for separating DNA fragments.
- **Option C: Blotting**: This is a crucial step in the Southern hybridization process.
- **Option D: Autoradiography**: This is also used to visualize the hybridized DNA.

4. **Conclusion**:
- Since PCR is not used in the Southern hybridization technique, the correct answer is **Option A: PCR**.

### Final Answer:
The analysis of chromosomal DNA using the Southern hybridization technique does not use **Option A: PCR**.