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(A) : Lysine (PI = 9.6) and glycine (PI ...

(A) : Lysine (PI = 9.6) and glycine (PI = 5.97) could be separated by electrophoresis
(R ) : In the electrophoresis process if the pH of the solution is maintained either at 9.6 or 5.97, one will migrate towards electric pole while other wouldn't.

A

Both (A) and (R ) are true and (R ) is the correct explanation of (A)

B

Both (A) and (R ) are true and (R ) is not the correct explanation of (A)

C

(A) is true but (R ) is false

D

(A) is false but (R ) is true

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question, we need to analyze the assertion (A) and the reason (R) provided regarding the separation of lysine and glycine by electrophoresis. ### Step-by-Step Solution: 1. **Understanding Isoelectric Point (pI)**: - The isoelectric point (pI) is the pH at which a particular molecule carries no net electrical charge. For lysine, the pI is 9.6, and for glycine, it is 5.97. 2. **Electrophoresis Basics**: - Electrophoresis is a technique used to separate charged particles in a solution using an electric field. The movement of these particles depends on their charge at a given pH. 3. **Effect of pH on Charge**: - At a pH below its pI, lysine (which is a basic amino acid) will carry a positive charge (cationic) and migrate towards the cathode (negative electrode). - At a pH above its pI, lysine will carry a negative charge and will not migrate. - Glycine, being a neutral amino acid at its pI (5.97), will have different behavior based on the pH: - Below its pI (like at pH 5.97), glycine will carry a positive charge and migrate towards the cathode. - Above its pI (like at pH 9.6), glycine will carry a negative charge (anionic) and migrate towards the anode (positive electrode). 4. **Analyzing the Statements**: - If the pH is set to 9.6: - Lysine will not migrate (as it is negatively charged). - Glycine will migrate towards the anode (as it is negatively charged). - If the pH is set to 5.97: - Lysine will migrate towards the cathode (as it is positively charged). - Glycine will not migrate (as it is neutral). 5. **Conclusion**: - At pH 9.6, glycine migrates while lysine does not. - At pH 5.97, lysine migrates while glycine does not. - Therefore, the assertion (A) is true, and the reason (R) correctly explains the assertion. ### Final Answer: Both the assertion (A) and reason (R) are true, and the reason is a correct explanation for the assertion.
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