A protein can be denaturated by ……….

Options

A. Change of oH
B. Addition of detergents
C. Adding Urea
D. Strong heating

Explanation

**Step-by-Step Solution:**

1. **Understanding Protein Denaturation:**
- Proteins are complex molecules that can lose their three-dimensional structure due to various environmental factors. This process is known as denaturation.

2. **Identifying Factors that Cause Denaturation:**
- Denaturation can occur due to several factors, including:
- Change in pH (acidic or alkaline conditions)
- Increased temperature (strong heating)
- Exposure to UV radiation or certain chemicals (like detergents or urea)

3. **Analyzing the Given Options:**
- The question provides four options:
1. Change of pH
2. Addition of detergent
3. Adding urea
4. Strong heating

4. **Evaluating Each Option:**
- **Change of pH:** This can lead to denaturation as it alters the ionic bonds and hydrogen bonds in the protein structure.
- **Addition of detergent:** Detergents can disrupt hydrophobic interactions, but they are not a primary cause of denaturation in the same way as pH changes.
- **Adding urea:** Urea can denature proteins by disrupting hydrogen bonds, but it is not as common as pH changes.
- **Strong heating:** High temperatures can cause proteins to denature by breaking the bonds that maintain their structure.

5. **Conclusion:**
- The most direct and common cause of protein denaturation among the options given is a change in pH. Therefore, the correct answer is **change of pH**.

**Final Answer:**
A protein can be denatured by **change of pH**.

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