Temperature required during autoclaving is

Options

A. `80^(@) C`
B. `100^(@) C`
C. `140^(@) C`
D. `121^(@) C`

Answer

To determine the temperature required during autoclaving, we can follow these steps:

### Step-by-Step Solution:

1. **Understand Autoclaving**:
- Autoclaving is a sterilization process that uses high pressure and temperature to eliminate microorganisms from equipment and culture media.

2. **Identify the Purpose of Autoclaving**:
- The primary purpose of autoclaving is to sterilize laboratory equipment, glassware, and culture media used in microbiology.

3. **Know the Conditions for Autoclaving**:
- Autoclaving requires specific conditions, including pressure and temperature. The standard pressure used is 15 psi (pounds per square inch).

4. **Determine the Temperature**:
- The temperature required for effective sterilization during autoclaving is 121 degrees Celsius. This temperature ensures that the saturated steam can penetrate and sterilize the items effectively.

5. **Evaluate the Given Options**:
- The options provided are:
- 80 degrees Celsius
- 100 degrees Celsius
- 140 degrees Celsius
- 121 degrees Celsius
- Among these, 121 degrees Celsius is the correct answer, as it is the standard temperature used in autoclaving.

### Final Answer:
The temperature required during autoclaving is **121 degrees Celsius**.

---