Nutrient medium is sterilized with the help of

Options

A. Laminar air flow chamber
B. Incubation chamber
C. Pasteurisation
D. Autoclave

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks about the method used to sterilize nutrient medium. Sterilization is essential to ensure that no unwanted microorganisms contaminate the medium.

2. **Analyzing the Options**:
- **Laminar Airflow Chamber**: This is a controlled environment used during experiments to prevent contamination, but it does not sterilize the medium itself.
- **Incubation Chamber**: This chamber is used to maintain specific temperatures for growing organisms but does not sterilize the medium.
- **Pasteurization**: This method involves heating to kill pathogens, commonly used for liquids like milk, but it is not specifically for sterilizing nutrient media.
- **Autoclave**: This is a device that uses steam under pressure to sterilize equipment and media effectively. It raises the temperature significantly, ensuring that all microorganisms are killed.

3. **Identifying the Correct Answer**: Among the options, the autoclave is the most effective method for sterilizing nutrient media. Therefore, the correct answer is **autoclave**.

4. **Conclusion**: Nutrient medium is sterilized with the help of an autoclave.

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