To isolate protoplast, one needs:

Options

A. pectinase
B. cellulase
C. both pectinasa and cellulase
D. chitinase

Answer

To isolate protoplasts, which are plant cells without cell walls, we need to break down the components of the cell wall. The cell wall is primarily composed of cellulose and pectin. To achieve this, specific enzymes are required to degrade these components.

### Step-by-Step Solution:

1. **Understand Protoplasts**: Protoplasts are plant cells that have had their cell walls removed. This is important for various applications in plant biotechnology, including somatic hybridization.

2. **Identify Cell Wall Components**: The cell wall of plant cells is mainly made up of cellulose and pectin. Therefore, to isolate protoplasts, we need to break down these components.

3. **Select Appropriate Enzymes**:
- **Cellulase**: This enzyme specifically breaks down cellulose, which is a major component of the plant cell wall.
- **Pectinase**: This enzyme breaks down pectin, another significant component of the cell wall.

4. **Combine Enzymes**: To effectively isolate protoplasts, both cellulase and pectinase are needed. This combination ensures that both cellulose and pectin are degraded, allowing the protoplasts to be released from the cell wall.

5. **Conclusion**: Therefore, to isolate protoplasts, one needs both pectinase and cellulase.

### Final Answer:
The correct answer is **Option C: both pectinase and cellulase**.

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