The nutrient culture medium is a misture of
I) Essential nutrients
II) Amino acids
(III) Vitamins
(IV) Carbohydrates

Options

A. I & II only correct
B. I, II, III, IV correct
C. I, II, III correct
D. I & IV correct

Answer

To determine the components of a nutrient culture medium, we can analyze each option provided in the question step by step.

### Step-by-Step Solution:

1. **Understanding Nutrient Culture Medium**:
- A nutrient culture medium is a growth medium used in laboratories for cultivating micro-organisms. It provides the necessary nutrients for their growth and reproduction.

2. **Identifying Components of Culture Medium**:
- The culture medium typically contains several essential components that support microbial life. These components include:
- Water
- Proteins
- Vitamins
- Carbohydrates
- Nitrogen sources
- Salts

3. **Evaluating Each Option**:
- **I) Essential Nutrients**: These are crucial for microbial growth and can be derived from sources like beef extract and meat extract. This option is correct.
- **II) Amino Acids**: Amino acids are the building blocks of proteins and are essential for the growth of micro-organisms. This option is also correct.
- **III) Vitamins**: Vitamins serve as coenzymes and are vital for various metabolic processes in micro-organisms. This option is correct as well.
- **IV) Carbohydrates**: Carbohydrates provide energy and are necessary for the growth of micro-organisms. This option is correct too.

4. **Conclusion**:
- Since all four options (I, II, III, IV) are components of the nutrient culture medium, the correct answer is that the nutrient culture medium is a mixture of all these components.

### Final Answer:
The nutrient culture medium is a mixture of:
- I) Essential nutrients
- II) Amino acids
- III) Vitamins
- IV) Carbohydrates

Thus, the answer is **option B: 1, 2, 3, 4**.

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