Basal medium does not esentially require
(I) Vitamins (II) NAA
(III) Cytokinins (IV) Carbohydrates

Options

A. I and IV
B. II and III
C. III and IV
D. I and IV

Answer

To solve the question regarding what is not essentially required in a basal medium, we can follow these steps:

### Step-by-Step Solution:

1. **Understand Basal Medium**:
- A basal medium is an unsupplemented growth medium that supports the growth of various organisms without the need for additional nutrient supplements.

2. **Analyze the Options**:
- **Option I: Vitamins** - These are essential nutrients that can promote growth in many organisms, including plants.
- **Option II: NAA (1-Naphthaleneacetic Acid)** - This is a synthetic plant hormone that belongs to the auxin family. It is not essential for basal medium.
- **Option III: Cytokinins** - These are another class of plant hormones that promote cell division and are not essential for basal medium.
- **Option IV: Carbohydrates** - These are crucial energy sources for organisms and are typically required in growth media.

3. **Determine Which Options are Not Essential**:
- From the analysis, both NAA (Option II) and Cytokinins (Option III) are not essential for a basal medium.
- Vitamins (Option I) and Carbohydrates (Option IV) are generally considered essential nutrients.

4. **Select the Correct Answer**:
- Since options II (NAA) and III (Cytokinins) are not essential for basal medium, the correct answer is **Option B: 2 and 3**.

### Final Answer:
**Option B: 2 and 3 (NAA and Cytokinins) do not essentially require in a basal medium.**

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