Identify the correct sequence of events involved in tissue culture experiments
(a) Preparation of explant
(b) Incubation for growth
(c) Preparation of nutrient culture medium
(d) Acclimatization of plantlets
(e) Innoculation of explant
(f) Sterilization of nutrient medium

Options

A. c, f, a, e, b, d
B. c, a, f, e, b, d
C. c, a, e, f, b, d
D. c, d, f, e, b, a

Answer

To identify the correct sequence of events involved in tissue culture experiments, we need to follow the logical steps involved in the process. Here’s the step-by-step solution:

1. **Preparation of Nutrient Culture Medium (c)**: The first step in tissue culture is to prepare a nutrient culture medium that provides the necessary nutrients for the growth of the plant tissue.

2. **Sterilization of Nutrient Medium (f)**: After preparing the nutrient medium, it must be sterilized to eliminate any contaminants that could affect the growth of the explant.

3. **Preparation of Explant (a)**: Once the nutrient medium is sterilized, the next step is to prepare the explant, which is the piece of plant tissue that will be cultured.

4. **Inoculation of Explant (e)**: After preparing the explant, it is inoculated into the sterile nutrient medium. This step involves placing the explant into the medium to initiate growth.

5. **Incubation for Growth (b)**: The inoculated explant is then incubated under controlled conditions (like temperature and light) to promote growth and development.

6. **Acclimatization of Plantlets (d)**: Finally, once the plantlets have developed, they need to be acclimatized, which means adapting them to the new environment before transferring them to soil or a different growth medium.

Putting it all together, the correct sequence of events is:
(c) Preparation of nutrient culture medium → (f) Sterilization of nutrient medium → (a) Preparation of explant → (e) Inoculation of explant → (b) Incubation for growth → (d) Acclimatization of plantlets.

### Final Sequence:
1. c - Preparation of nutrient culture medium
2. f - Sterilization of nutrient medium
3. a - Preparation of explant
4. e - Inoculation of explant
5. b - Incubation for growth
6. d - Acclimatization of plantlets