Which of the following stain is used to observe mitochondria?

Options

A. Methylene blue
B. Safranin
C. Janus green
D. Gram stain

Answer

To determine which stain is used to observe mitochondria, we can follow these steps:

### Step-by-Step Solution:

1. **Understand Mitochondria**:
- Mitochondria are organelles found in cells that are primarily responsible for energy production. They convert nutrients into adenosine triphosphate (ATP), which is the energy currency of the cell.

**Hint**: Recall that mitochondria are often referred to as the "powerhouse of the cell."

2. **Identify Stains**:
- We need to look at the options provided and understand what each stain is typically used for.

3. **Evaluate Each Option**:
- **Option A: Methylene Blue**:
- This stain is primarily used to stain bacterial cells. Therefore, it is not suitable for staining mitochondria.
- **Option B: Safranin**:
- Safranin is commonly used to stain plant cells, particularly lignified tissues like xylem. This makes it an incorrect choice for staining mitochondria.
- **Option C: Janer's Green**:
- Janer's Green is specifically used to stain mitochondria, making it the correct choice.
- **Option D: Gram Stain**:
- Gram stain is used to identify bacterial infections and is not used for mitochondria.

4. **Conclusion**:
- After evaluating all options, the correct answer is **Option C: Janer's Green**.

### Final Answer:
The stain used to observe mitochondria is **Janer's Green**.