Home
Class 12
BIOLOGY
In their semiconservative experiment Mes...

In their semiconservative experiment Meselson and Stahl used

A

`""^(14)C `

B

`""^(32)P`

C

`""^(15)N`

D

`""^(35)S`

Text Solution

AI Generated Solution

The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding the Experiment**: Meselson and Stahl conducted an experiment to demonstrate the semi-conservative nature of DNA replication. This means that when DNA replicates, each new DNA molecule consists of one old strand and one newly synthesized strand. 2. **Centrifugation Method**: They used a technique called density gradient centrifugation to separate DNA based on its density. This method allows for the separation of different DNA molecules that have varying densities. 3. **Use of Isotopes**: In their experiment, Meselson and Stahl cultured bacteria in a medium containing a heavy isotope of nitrogen, specifically Nitrogen-15 (N15). This heavy isotope was incorporated into the DNA of the bacteria. 4. **Heavy Density of DNA**: Because the DNA synthesized in the presence of N15 had a heavier density compared to normal DNA (which contains the lighter isotope Nitrogen-14), it allowed for the differentiation of DNA strands during centrifugation. 5. **Conclusion**: After several rounds of replication, the DNA was analyzed, and the results confirmed that the DNA replication was semi-conservative. The correct answer to the question is that Meselson and Stahl used Nitrogen-15 in their experiment. **Final Answer**: In their semi-conservative experiment, Meselson and Stahl used Nitrogen-15 (N15). ---
Promotional Banner

Topper's Solved these Questions

  • MOLECULAR BASIS OF INHERITANCE

    AAKASH SERIES|Exercise Exercise-I (Transcription)|36 Videos
  • MOLECULAR BASIS OF INHERITANCE

    AAKASH SERIES|Exercise Exercise-I (Genetic Code)|23 Videos
  • MOLECULAR BASIS OF INHERITANCE

    AAKASH SERIES|Exercise Exercise-I (The search for genetic material)|14 Videos
  • MINERAL NUTRITION

    AAKASH SERIES|Exercise Exercise - III|52 Videos
  • NEURAL CONTROL AND COORDINATION

    AAKASH SERIES|Exercise EXERCISE-III( PREVIOUS AIPMT / NEET QUESTIONS)|33 Videos

Similar Questions

Explore conceptually related problems

Isotopes used for proving semiconservative replication of DNA by Meselson and Stahl are

Watson and Crick (1953) proposed DNA was based on (i) X-ray diffraction studies studies of DNA dne by Wilkins and Franklin (ii) Chargaff's base equivalence rule (iii)Griffith's transformation experiment (iv) Meselson and Stahl's experiment.

DNA in chromosome replicate semiconservatively was experimently proved in vicia faba by _________using_______.

Answer the following questions based on Meselson and Stahl s experiment: (a) Write the name of the chemical substance used as a source of nitrogen in the experiment by them. (b) Why did the scientists synthesise the light and the heavy DNA molecules In the organism used in the experiment ? (c) How did the scientists make it possible to distinguish the heavy DNA molecule from the light DNA molecule ? Explain. ((1) Write the conclusion the scientists arrived at after completing the experiment.

Used in cryoscopic experiment

Which of the following phenomena was experimentally proved by Meselson and Stahl?

Answer the following questions based on Meselson and Stahl's experiment of E. coli : (a) Write the name of the chemical substance used as the only source of nitrogen in the experiment. (b) Why did they allow the synthesis of the light and the heavy DNA molecules in the organism ? (c ) How did they distinguish the heavy DNA molecules from the light DNA molecules? Explain. (d) Write the conclusion the scientists arrived at, at the end of the experiment.

Hertz experiment is used for

Go though the following statements (i) Meselson & stahl used cesium chloride density gradient to distinguish heavy DNA molecules from the normal DNA molecules. (ii) In meselson & Stahl experiment the DNA that was extracted from the culture one generation after the transfer from .^(15)N to .^(14)N , was composed of equal amount of hybrid (intermediate) DNA and of light DNA. (iii) In prokaryotes, control the rate of transcriptional initiation is the predominant site for control of gene expression. (iv) In lac operon, the repressor is synthesised only at the time when the level of metabolite is in excess. Find out the correct statements?

Fungus used in genetic experiments is