During DNA finger printing, the double stranded DNA is split into single stranded DNA by using

Options

A. agarose gel
B. radioactive DNA probe
C. acidic chemicals
D. alkaline chemical

Answer

**Step-by-Step Solution:**

1. **Understanding DNA Fingerprinting**: DNA fingerprinting is a technique used to identify individuals based on their unique DNA profiles. It involves analyzing specific regions of DNA that vary greatly among individuals.

2. **The Process of DNA Denaturation**: In DNA fingerprinting, the first step is to separate the double-stranded DNA (dsDNA) into single-stranded DNA (ssDNA). This process is known as DNA denaturation.

3. **Role of Chemicals in Denaturation**: DNA denaturation can be achieved using certain chemicals. Specifically, alkaline chemicals are commonly used to break the hydrogen bonds between the complementary bases of the DNA strands, leading to the separation of the strands.

4. **Evaluating the Options**:
- **Agarose Gel**: This is used for the separation of DNA fragments during electrophoresis, not for denaturation.
- **Radioactive DNA Probe**: This is used in hybridization techniques to detect specific DNA sequences but does not denature DNA.
- **Acidic Chemicals**: These are generally used to wash out hybridized probes and do not play a role in DNA denaturation.
- **Alkaline Chemicals**: These are the correct choice as they are specifically used to denature DNA by breaking the hydrogen bonds between the strands.

5. **Conclusion**: Therefore, the correct answer to the question is that during DNA fingerprinting, the double-stranded DNA is split into single-stranded DNA using alkaline chemicals.

**Final Answer**: The double-stranded DNA is split into single-stranded DNA by using **alkaline chemicals**.

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