The process of transfer of DNA pieces on to the surface of the nylone membrane is called

Options

A. blotting
B. gel electrophoresis
C. autoradiography
D. isolation of DNA from the sample

Answer

To answer the question about the process of transferring DNA pieces onto the surface of a nylon membrane, we can break it down into the following steps:

### Step-by-Step Solution:

1. **Understanding the Context**: The question is related to molecular biology techniques used in DNA analysis, specifically in DNA fingerprinting.

2. **Identifying the Techniques**: In DNA fingerprinting, various techniques are employed to manipulate and analyze DNA. The process in question involves transferring DNA fragments.

3. **Recognizing Gel Electrophoresis**: Before the transfer, DNA fragments are typically separated using gel electrophoresis. This technique allows for the separation of DNA based on size.

4. **Blotting Technique**: After gel electrophoresis, the next step is to transfer the separated DNA fragments onto a membrane. This process is known as "blotting."

5. **Types of Blotting**: The specific type of blotting that transfers DNA onto a nylon membrane is often referred to as "Southern blotting." This technique is named after the scientist Edwin Southern, who developed it.

6. **Conclusion**: Therefore, the process of transferring DNA pieces onto the surface of the nylon membrane is called "blotting."

### Final Answer:
The process of transfer of DNA pieces onto the surface of the nylon membrane is called **blotting**.

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