Which of these statements is incorrect?

Options

A. Enzymes of TCA cycle are present in mitochondrial matrix
B. Oxidative phosphorylation takes place in outer mitochondrial membrane
C. Glycolysis operates as long as it is supplied with `NAD^+` that can pick up hydrogen atoms
D. Glycolysis occurs in cytosol

Answer

To determine which statement is incorrect, let's analyze each option step by step:

1. **Option 1: Enzymes of TCA Cycle are present in mitochondrial matrix.**
- The TCA Cycle, also known as the Krebs Cycle or Citric Acid Cycle, occurs in the mitochondrial matrix. This statement is true.

2. **Option 2: Oxidative phosphorylation takes place in the outer mitochondrial membrane.**
- Oxidative phosphorylation is the process where ATP is produced through the electron transport chain and chemiosmosis, which occurs in the inner mitochondrial membrane, not the outer mitochondrial membrane. Therefore, this statement is incorrect.

3. **Option 3: Glycolysis operates as long as it is supplied with NAD+.**
- Glycolysis requires NAD+ to accept electrons and convert into NADH. Without NAD+, glycolysis cannot proceed. This statement is true.

4. **Option 4: Glycolysis occurs in cytosol.**
- Glycolysis is indeed a metabolic pathway that occurs in the cytosol (cytoplasm) of the cell. This statement is true.

Based on the analysis, the incorrect statement is **Option 2: Oxidative phosphorylation takes place in the outer mitochondrial membrane.**