Transformation is
(A) Insertion of gene into suitable vector
(B) Transfer of chimeric DNA into host cell
(C) Synthesis of polypeptide chain from m-RNA
(D) Synthesis of C-DNA from RNA
A
B
C
D

Synthesis of DNA from RNA is explained by

Which one of the following enzyme is responsible for the synthesis of DNA from RNA

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Hybridomas are employed for (a) Production of somatic hybrids (b) Killing cancer cells (c) Synthesis of antibiotics (d) Synthesis of monoclonal antibodies

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

Rearrrange the following in the correct sequence to accomplish an important biotechnological reaction: ( a) In vitro synthesis of copies of DNA of interest (b) Chemically synthesized oligo - nucleqtides ( c) Enzyme DNA - polymerase ( d) Complementary region of DNA ( e) Gernomic DNA template (f) Nuceltides provided ( g)Primes (h) Thermostable DNA -polymerase (from Thermus aquaticus ) ( i) Denaturation of ds- DNA

The following features occur during protein synthesis: (i) Movement of m-RNA from the nucleus into the Cytoplasm, where it binds to the ribosomes (ii) Formation of amino acyl t-RNA complex (iii) Transcription of specific segments of DNA into m-RNA molecules in the nucleus (iv) Binding of N-terminal amino acid, methionine t-RNA to the 'P' site of the ribosome. The t-RNA anticodon "pairs with AUG on m-RNA" (v) Formation of peptide bond between first and second amino acids at the 'A' site (vi) Release of the completed polypeptide chain from ribosomes (vii) Migration of dipeptide to form a tripeptide and then a polypeptide The correct order of events is

There are three basic steps in creating genetically modified organisms. Arrange these steps in the correct sequence. (a.) Introduction of the identified DNA into the host (b.) Maintenance of introduced DNA in the host and transfer of the DNA to its progeny (c.) Identification of DNA with desirable genes

Agrobacterium tumefaciens : (I) has earned the name of natural genetic engineer for the bacterium (II) carries a vir gene which promotes insertion of T-DNA into host cell . (III) has been used to transfer genes for RNA interfer- ence in plants

The photochemical phase of photosynthesis includes (a) Water splitting (b) Light absorption (c) Oxygen release (d) Synthesis of NADPH (e) Synthesis of starch