In selection of recombinant by inactivation of lac `Z^1` gene the common vectors used are

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

A: Selection of recombinants due to inactivation of antibiotics is cumbersome procedure. R: It requires simultaneous plating on two plates having different antibiotics.

A: Selection of recombinants due to inactivation of antibiotics is cumbersome procedure. R: It requires simultaneous plating on two plates having different antibiotics.

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

Study the schematic representation of the genes involved in the lac operon given below and answer the questions that follow : {:(p,i,p,o,z,y,a):} (a) Identify and name the regulatory gene in this operon. Explain its role in 'switching off' the operon. (b) Why is the lac operon's regulation referred to as negative regulation ? (c ) Name the inducer molecule and the products of the genes 'z' and 'y' of the operon. Write the functions of these gene products.

Blue-white reaction is used as a method of selection of recombinants using the following substrate:

A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e., bacterial transformation?

Read the following statements for lac operon: i. Each operon has its specific operator and specific repressor. ii. The y gene codes permease, which increases the permeability of the cell to beta-galactosides. iii. Regulation of lac operon can be visualized as regulation of enzyme synthesis by its substrate. Select the option with the correct statements.