In rice genome project large insert genomic libraries used as the primary sequencing templates are costructed in

Options

A. Bacterisal temporary chromosomes
B. Yeast artificial chromosomes
C. bacterial artificical chromosomes
D. yeast mechanical chromosomes

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks about the type of large insert genomic libraries used as primary sequencing templates in the Rice Genome Project.

2. **Identifying the Key Terms**: The key terms in the question are "large insert genomic libraries" and "Rice Genome Project". This indicates that we are looking for a specific method or technology used in genomic sequencing.

3. **Recalling Relevant Information**: In genomic projects, especially those involving large insert libraries, various cloning vectors are used. The most common types include Bacterial Artificial Chromosomes (BACs), Yeast Artificial Chromosomes (YACs), and others.

4. **Analyzing the Options**:
- Bacterial Artificial Chromosomes (BACs) are known for their ability to clone large fragments of DNA (up to 300 kb), making them suitable for genomic libraries.
- Yeast Artificial Chromosomes (YACs) can also clone large DNA fragments but are less commonly used due to stability issues.
- Other methods like mechanical chromosomes are not typically used for constructing genomic libraries.

5. **Conclusion**: Based on the information gathered, the primary sequencing templates used in the Rice Genome Project were constructed using Bacterial Artificial Chromosomes (BACs).

**Final Answer**: In the Rice Genome Project, large insert genomic libraries used as primary sequencing templates are constructed in Bacterial Artificial Chromosomes (BACs).

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