Take a clean glass slide and put few drops of water on it. Now place a complete Rhea leaf on water droplets and examine the cells of leaf under the high power of compound microscope. Put a few drops of concentrated salt/sugar solution on the mounted Rhea leaf on the glass slide. Wait for few minutes and again observe the leaf under the high power of microscope.
What will be your observation after few minutes?

Options

A. Cell contents are separated from the cell wall.
B. Cytoplasm along with plasma membrane has come to lie on one side of cell wall.
C. A clear space is seen between the cell wall and protoplast of the cell.
D. All of these

Explanation

### Step-by-Step Text Solution:

1. **Preparation of the Slide**:
- Take a clean glass slide and place a few drops of water on it. This creates a medium for the leaf to be placed on.

2. **Mounting the Leaf**:
- Carefully place a complete Rhea leaf on the water droplets. Ensure that the leaf is flat and in contact with the water.

3. **Initial Observation**:
- Use a high power compound microscope to observe the cells of the Rhea leaf. At this stage, you will see the cells with their contents, including the cytoplasm and the cell wall.

4. **Adding Concentrated Solution**:
- After the initial observation, put a few drops of concentrated salt or sugar solution on the mounted Rhea leaf. This solution is hypertonic, meaning it has a higher concentration of solutes compared to the inside of the leaf cells.

5. **Waiting Period**:
- Allow the slide to sit for a few minutes. This waiting period is essential for the process of osmosis to occur.

6. **Final Observation**:
- After a few minutes, observe the leaf again under the microscope. You will notice that the cells have undergone a process called plasmolysis. The cytoplasm will have shrunk away from the cell wall, and there will be a clear space between the cell wall and the cytoplasm.

7. **Conclusion**:
- The observation indicates that water has moved out of the cells into the hypertonic solution due to osmosis, causing the cytoplasm to shrink and pull away from the cell wall.

### Observations:
- The cell wall remains intact, but the cytoplasm is separated from it.
- A clear space is visible between the cell wall and the cytoplasm.
- This phenomenon is known as plasmolysis.