DNA

Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

Arrange the various steps of DNA fingerprinting technique in the correct order. (i) Separation of DNA fragments by electrophoresis. (ii) Digestion of DNA by restriction endonucleases. (iii) Hybridisation using labelled VNTR prode. (iv) Isolation of DNA . Detection of hybridised DNA fragments by auto-radiography. (vi) Transferring the separated DNA fragments to nitrocellulose membrane.