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DNA moves towards …… during agarose gel ...

DNA moves towards …… during agarose gel electrophoresis.

A

negative terminal

B

cathode

C

anode

D

does not move

Text Solution

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The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding Agarose Gel Electrophoresis**: Agarose gel electrophoresis is a technique used to separate DNA fragments based on their size. The gel acts as a medium through which the DNA can migrate when an electric current is applied. 2. **Charge of DNA**: DNA molecules inherently possess a negative charge due to the phosphate groups in their backbone. This negative charge is crucial for their movement in an electric field. 3. **Setup of the Electrophoresis Apparatus**: In the electrophoresis setup, the agarose gel is placed in a tank with two electrodes: the anode (positive electrode) and the cathode (negative electrode). The wells containing the DNA samples are typically placed near the cathode. 4. **Application of Electric Current**: When an electric current is applied, the negatively charged DNA molecules will move towards the positively charged electrode (anode) because opposite charges attract. 5. **Direction of DNA Movement**: Since DNA is negatively charged, it will migrate towards the anode during the electrophoresis process. 6. **Conclusion**: Therefore, the correct answer to the question "DNA moves towards … during agarose gel electrophoresis" is "anode". **Final Answer**: DNA moves towards the anode during agarose gel electrophoresis. ---
**Step-by-Step Solution:** 1. **Understanding Agarose Gel Electrophoresis**: Agarose gel electrophoresis is a technique used to separate DNA fragments based on their size. The gel acts as a medium through which the DNA can migrate when an electric current is applied. 2. **Charge of DNA**: DNA molecules inherently possess a negative charge due to the phosphate groups in their backbone. This negative charge is crucial for their movement in an electric field. 3. **Setup of the Electrophoresis Apparatus**: In the electrophoresis setup, the agarose gel is placed in a tank with two electrodes: the anode (positive electrode) and the cathode (negative electrode). The wells containing the DNA samples are typically placed near the cathode. ...
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