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EcoRI is used to cut a segment of foreig...

EcoRI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams and answer the following questions.

EcoRI cuts the DNA between the bases. What does EcoRI stand for?

Text Solution

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Eco stands for Escherichia coli, R stands for the name of the strain and Roman I indicates the order in which the enzymes were isolated from the strains of the bacteria.
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EcoR I cut the DNA between the bases

Eco RI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams. (i) The set of palindronic nucleotide sequence of baes pairs the Eco RI will recognise in both the DNA segment. Mark the site at which Eco RI will act and cut both the segments. (ii) Sticky ends of formed on both the segments where the two DNA segments will join later to form a recombinant DNA.

Restriction enzyme Eco RI cuts the DNA between bases G and A only when the sequence in DNA is

A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined.

Draw schematic diagrams of segments of a vector and a foreign DNA with the sequence of nucleotides recognised by EcoRI.

The specific DNA sequence where EcoRI cuts is