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EcoRI is used to cut a segment of foreig...

EcoRI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams and answer the following questions.

What are sticky ends?

Text Solution

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They are the resultant of hydrogen bonding that act as the site for attachment for the separated DNA fragments and facilitate the action of enzyme called DNA ligase.
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Eco RI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams. (i) The set of palindronic nucleotide sequence of baes pairs the Eco RI will recognise in both the DNA segment. Mark the site at which Eco RI will act and cut both the segments. (ii) Sticky ends of formed on both the segments where the two DNA segments will join later to form a recombinant DNA.

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