Bioreactor: A bioreactor is a vessel in which a biological reaction or change takes place. The biological systems involved include enzymes, microorganisms, animal cells, plant cells, and tissues.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
Bioreactor: A bioreactor is a vessel in which a biological reaction or change takes place. The biological systems involved include enzymes, microorganisms, animal cells, plant cells, and tissues.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
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1. **Understanding the Bioreactor**: A bioreactor is a vessel designed to support a biological reaction. It can involve various biological systems such as enzymes, microorganisms, animal cells, plant cells, and tissues.
2. **Introduction to Agarose Gel Electrophoresis**: This technique is used to separate DNA molecules. It involves placing DNA samples into wells of an agarose gel and applying an electric current.
3. **Charge of DNA**: DNA molecules are negatively charged due to their phosphate backbone. When an electric current is applied, the negatively charged DNA will migrate towards the positive electrode (anode).
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Read the passage given below and answer the question: Biopolymers are polymers that are generated from renewable natural sources, are often biodegradable and nontoxic. They can be produced by biological systems (i.e. microorganisms, plants and animals), or chemically synthesized from biological materials (e.g., sugars, starch, natural fats or oils, etc.). Two strategies are applied in converting these raw materials into biodegradable polymers: extraction of the native polymer from a plant or animal tissue, and a chemical or biotechnological route of monomer polymerization. Biodegradable biopolymers (BDP) are an alternative to petroleum-based polymers (traditional plastics). Some BDP degrade in only a few weeks, while the degradation of others takes several months. In principle the properties relevant for application as well as biodegradability are determined by the molecular structure. According to the American Society for Testing and Materials, biopolymers are degradable polymers in which degradation results from the action of naturally occurring microorganisms such as bacteria, fungi and algae. Polylactic acid (PLA) is an example of biopolymer. It is a thermoplastic polyester. Generally, there are two major routes to produce polylactic acid from the lactic acid (CH_(3)CH(OH) COOH) monomer. The first route involves condensation-water removal by the use of solvent under high vacuum and temperature. This approach produces a low to intermediate molar mass polymer. An alternative method is to remove water under milder conditions, without solvent, to produce a cyclic intermediate dimer, referred to as lactide. This intermediate is readily purified by vacuum distillation. Ring opening polymerization of the dimer is accomplished under heat, again without the need for solvent. By controlling the purity of the dimer it is possible to produce a wide range of molar masses .PLA is a good material for production of clothing, carpet tiles, interior and outdoor furnishing, geotextiles, bags, filtration systems, etc. The primary biodegradability of PLA was tested using hydrolysis tests at various composting temperatures and pH. It was demonstrated that composting is a useful method for PLA biodegradation. The degradation rate is very slow in ambient temperatures. A 2017 study found that at 25 ^(@)C in sea water, PLA showed no degradation over a year. As a result, it is poorly degraded in landfills and household composts, but is effectively digested in hotter industrial composts. (source: Flieger, M., Kantorová, M., Prell, A., Rezanka, T., & Votruba, J. (2003). Biodegradable plastics from renewable sources. Folia Microbiologica, 48(1), 27 44. doi:10.1007/bf02931273) In the question, a statement of assertion followed by a statement of reason is given. Choose the correct answer out of the following choices on the basis of the above passage. Assertion: PLA is poorly degraded in landfills. Reason: The degradation rate of PLA is very slow in ambient temperatures.
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Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.
Read the following statements (a-e) and choose the suitable option w.r.t. respiration a. A series of slow step-wise reactions controlled by enzymes. b. Breaking of the C-C bonds of respiratory substrates through oxidation within the cells. c. Liberation of energy. d. Breakdown of complex molecules to yield energy which takes place in mitochondria only. e. Carbon skeleton produced during respiration is not used as precursor for cellular biosynthesis of other molecules.
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Assertion: A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions. Reason :Bioreactors are vessels in which raw materials are biologically converted into specific products using microbial, plant or animal cells
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