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The image below depicts the result of ge...

The image below depicts the result of gel electrophoresis

If the ladder represents sequence length upto 3000 base pairs (bp),
Explain the basis of this kind of separation and also mention the significance of this process.

Text Solution

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The fragments will resolve according to their size. The shorter sequence fragments would move farthest from well as seen in Band IV (100 bp) which is lighter as compared to Band III which is heavier being 2500 base pairs. The significance of electrophoresis is to purify the DNA fragments for use in constructing recombinant DNA by joining them with cloning vectors.
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