Home
Class 12
BIOLOGY
Some restriction enzymes break a phospho...

Some restriction enzymes break a phosphodiester bond on both the DNA strands, such that only one end of each molecule is cut and these ends have regions of single stranded DNA. BamH1 is one such restriction enzyme which binds at the recognition sequence, 5'-GGATCC-3' and cleaves these sequences just after the 5'- guanine on each strand.
What is the objective of this action?

Text Solution

Verified by Experts

The two different DNA molecules will have compatible ends to recombine when cut with same restriction enzymes.
Promotional Banner

Similar Questions

Explore conceptually related problems

If one strand of DNA has the nucleotide sequence 5' GATCAA 3', its complementary strand will have the sequence :

The 'sticky ends' on each of the two strands of a DNA generated by treatement with restriction enzyme facilitate the action of enzyme :

Which of the following is an enzyme used to form a phosphodiester bond in link between a 3' end of one DNA chain and 5' end of another ?

When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, so that the DNA fragments have single-stranded ends. These ends are useful in recombinant DNA work because

When a typical restriction enzyme cuts a DNA moleule, the cuts are uneven so that the DNA fragments have single-stranded ends . These ends are useful in recombinant DNA work because :

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA polymerase

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickness of the ends facilitates the action of the enzyme DNA polymerase