Some restriction enzymes break a phosphodiester bond on both the DNA strands, such that only one end of each molecule is cut and these ends have regions of single stranded DNA. BamH1 is one such restriction enzyme which binds at the recognition sequence, 5'-GGATCC-3' and cleaves these sequences just after the 5'- guanine on each strand.
Explain how the gene of interest is introduced into a vector.

Explanation

To introduce a gene of interest (GOI) into a vector using a restriction enzyme like BamH1, follow these steps:

### Step-by-Step Solution:

1. **Selection of Vector and Gene of Interest**:
- Choose an appropriate vector (e.g., plasmid like pBR322) that contains a restriction site for BamH1.
- Identify and isolate the gene of interest (GOI) that you want to clone.

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