Some restriction enzymes break a phosphodiester bond on both the DNA strands, such that only one end of each molecule is cut and these ends have regions of single stranded DNA. BamH1 is one such restriction enzyme which binds at the recognition sequence, 5'-GGATCC-3' and cleaves these sequences just after the 5'- guanine on each strand.
A gene Mwas introduced into E.coli cloning vector PBR322 at BamH1 site. What will be its impact on the recombinant plasmids? Give a possible way by which you could differentiate non recombinant to recombinant plasmids.

Explanation

To solve the question, we will break it down into steps:

### Step 1: Understanding the Role of BamH1
BamH1 is a restriction enzyme that recognizes the specific DNA sequence 5'-GGATCC-3' and cleaves the DNA just after the first guanine (G) on each strand. This creates sticky ends with single-stranded overhangs.

### Step 2: Introduction of Gene M into PBR322
When the gene M is introduced into the E. coli cloning vector PBR322 at the BamH1 site, it replaces the sequence that would normally be present at that site. This insertion of foreign DNA (gene M) into the plasmid results in the formation of recombinant plasmids.

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