Gene of interest/alien gene is introduced by a cloning vector into a host cell to bring about a desired phenotypic expression in a host cell. The cloning vectors used are plasmid and bacteriophages. Biotechnologists in their labs, for desired results engineered specialised cloning vectors. One such vector is PBR322. Study the diagram carefully and answer the questions that follow.

What do 'EcoR I, 'BamH I' and 'Hind III represent ? State their functions.
(ii) Identify the gene you would select for the role of a selectable marker in PBR322. Explain why.
(iii) Write the property/characteristic of plasmid and bacteriophage that makes them efficient cloning vectors .
(iv) Biotechnologists always insert 'ori' gene in their engineered cloning vector. Justify the statement.
(v) Will the experiment be successful if the alien DNA is ligated at Hind III restriction site ? Give reason in support of your.