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Gene of interest/alien gene is introduce...

Gene of interest/alien gene is introduced by a cloning vector into a host cell to bring about a desired phenotypic expression in a host cell. The cloning vectors used are plasmid and bacteriophages. Biotechnologists in their labs, for desired results engineered specialised cloning vectors. One such vector is pBR322. Study the diagram carefully and answer the questions that follow.

Biotechnologists always insert ‘ori' gene in their engineered cloning vector. Justify the statement.

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