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Gene of interest/alien gene is introduce...

Gene of interest/alien gene is introduced by a cloning vector into a host cell to bring about a desired phenotypic expression in a host cell. The cloning vectors used are plasmid and bacteriophages. Biotechnologists in their labs, for desired results engineered specialised cloning vectors. One such vector is pBR322. Study the diagram carefully and answer the questions that follow.

Will the experiment be successful if the alien DNA is ligated at Hind III restriction site ? Give reason in support of your answer.

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