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Electron microscope is used for...

Electron microscope is used for

A

Viewing structure of the cell

B

Whole-mount study

C

Cell division study

D

Structure of the pollen grain

Text Solution

Verified by Experts

The correct Answer is:
A
Electron microscope is used to view thin specimens (tissue sections, molecules) through which electrons can pass generating a projection image.
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Read the following text and answer the following questions on the basis of the same: Electron Microscope Electron microscopes use electrons to illuminate a sample. In Transmission Electron Microscopy (TEM), electrons pass through the sample and illuminate film or a digital camera. Resolution in microscopy is limited to about half of the wavelength of the illumination source used to image the sample. Using visible light the best resolution that can be achieved by microscopes is about -200nm . Louis de Broglie showed that every particle or matter propagates like a wave. The wavelength of propagating electrons at a given accelerating voltage can be determined by l= (h)/(sqrt(2m_(e )v)) . Thus the wavelength of electrons is calculated to be 3.88pm when the microscope is operated at 100keV, 2.74pm at 200keV and 2.24pm at 300keV. However, because the velocities of electrons in an electron microscope reach about 70% the speed of light with an accelerating voltage of 200keV, there are relativistic effects on these electrons. Due to this effect, the wavelength at 100keV, 200keV and 300keV in electron microscopes is 3.70pm, 2.51 pm and 1.96pm, respectively. Anyhow, the wavelength of electrons is much smaller than that of photons (2.5pm at 200keV). Thus if electron wave is used to illuminate the sample, the resolution of an electron microscope theoretically becomes unlimited. Practically, the resolution is limited to ~-0.1 nm due to the objective lens system in electron microscopes. Thus, electron microscopy can resolve subcellular structures that could not be visualized using standard fluorescences microscopy. In electorn microscope, electron is used

Read the following text and answer the following questions on the basis of the same: Electron Microscope Electron microscopes use electrons to illuminate a sample. In Transmission Electron Microscopy (TEM), electrons pass through the sample and illuminate film or a digital camera. Resolution in microscopy is limited to about half of the wavelength of the illumination source used to image the sample. Using visible light the best resolution that can be achieved by microscopes is about -200nm . Louis de Broglie showed that every particle or matter propagates like a wave. The wavelength of propagating electrons at a given accelerating voltage can be determined by l= (h)/(sqrt(2m_(e )v)) . Thus the wavelength of electrons is calculated to be 3.88pm when the microscope is operated at 100keV, 2.74pm at 200keV and 2.24pm at 300keV. However, because the velocities of electrons in an electron microscope reach about 70% the speed of light with an accelerating voltage of 200keV, there are relativistic effects on these electrons. Due to this effect, the wavelength at 100keV, 200keV and 300keV in electron microscopes is 3.70pm, 2.51 pm and 1.96pm, respectively. Anyhow, the wavelength of electrons is much smaller than that of photons (2.5pm at 200keV). Thus if electron wave is used to illuminate the sample, the resolution of an electron microscope theoretically becomes unlimited. Practically, the resolution is limited to ~-0.1 nm due to the objective lens system in electron microscopes. Thus, electron microscopy can resolve subcellular structures that could not be visualized using standard fluorescences microscopy. Who showed that electron also propagates like a wave?

Read the following text and answer the following questions on the basis of the same: Electron Microscope Electron microscopes use electrons to illuminate a sample. In Transmission Electron Microscopy (TEM), electrons pass through the sample and illuminate film or a digital camera. Resolution in microscopy is limited to about half of the wavelength of the illumination source used to image the sample. Using visible light the best resolution that can be achieved by microscopes is about -200nm . Louis de Broglie showed that every particle or matter propagates like a wave. The wavelength of propagating electrons at a given accelerating voltage can be determined by l= (h)/(sqrt(2m_(e )v)) . Thus the wavelength of electrons is calculated to be 3.88pm when the microscope is operated at 100keV, 2.74pm at 200keV and 2.24pm at 300keV. However, because the velocities of electrons in an electron microscope reach about 70% the speed of light with an accelerating voltage of 200keV, there are relativistic effects on these electrons. Due to this effect, the wavelength at 100keV, 200keV and 300keV in electron microscopes is 3.70pm, 2.51 pm and 1.96pm, respectively. Anyhow, the wavelength of electrons is much smaller than that of photons (2.5pm at 200keV). Thus if electron wave is used to illuminate the sample, the resolution of an electron microscope theoretically becomes unlimited. Practically, the resolution is limited to ~-0.1 nm due to the objective lens system in electron microscopes. Thus, electron microscopy can resolve subcellular structures that could not be visualized using standard fluorescences microscopy. As the accelerating voltage increases, the wavelength of electron as wave