The enzymes required to obtain protoplasts are

Options

A. Cellulase and proteinase
B. Cellulase and amylase
C. Cellulase and pectinase
D. Amylase and pectinase

Explanation

To answer the question about the enzymes required to obtain protoplasts, we can follow these steps:

### Step 1: Understand what protoplasts are
Protoplasts are plant cells that have had their cell wall removed, leaving only the cell membrane and the contents of the cell (cytoplasm and organelles). This process is essential for various biological experiments and applications.

### Step 2: Identify the components of the plant cell wall
The plant cell wall is primarily composed of cellulose, pectin, and other polysaccharides. To obtain protoplasts, the cell wall must be broken down.

### Step 3: Determine the enzymes that can break down the cell wall components
The enzymes that are commonly used to degrade the components of the cell wall include:
- **Cellulase**: This enzyme breaks down cellulose into smaller sugars.
- **Pectinase**: This enzyme breaks down pectin, which is another major component of the plant cell wall.

### Step 4: Recognize the role of other enzymes
While other enzymes like proteinase (which breaks down proteins) and amylase (which breaks down starch) are important in different contexts, they are not specifically required for the breakdown of the cell wall to obtain protoplasts.

### Step 5: Conclude with the correct answer
Based on the above understanding, the enzymes required to obtain protoplasts are **cellulase and pectinase**.

### Final Answer:
The enzymes required to obtain protoplasts are cellulase and pectinase.

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