Master plate in replica plating experiment contained the

Options

A. Drug resistant bacteria
B. Sterile colony of bacteria
C. Different colonies of fertile bacteria
D. None of the above

Explanation

**Step-by-Step Solution:**

1. **Understanding the Replica Plating Experiment:**
- The replica plating experiment is a technique used to identify and isolate specific bacterial colonies based on their characteristics, such as antibiotic resistance.

2. **Identifying the Master Plate:**
- The master plate is the original plate where various bacterial colonies are grown. It contains different types of bacteria, including those that are antibiotic-resistant and those that are not.

3. **Process of Replica Plating:**
- In the experiment, a cloth is used to stamp the colonies from the master plate and then stamp them onto two new plates: one with a normal medium and one with a medium containing an antibiotic.

4. **Observing Growth on Plates:**
- On the normal medium plate, all colonies from the master plate grow as expected.
- On the antibiotic medium plate, only the colonies that are resistant to the antibiotic will survive and grow.

5. **Conclusion about the Master Plate:**
- Since the master plate contains various colonies of bacteria, including those that are antibiotic-resistant, the correct answer to the question is that the master plate contained "different colonies of fertile bacteria."

**Final Answer:**
The master plate in the replica plating experiment contained different colonies of fertile bacteria.

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