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Which of the given statements is correct...

Which of the given statements is correct in the context of observing DNA separated by agarose gel electrophoresis ?

A

DNA can be seen in visible light

B

DNA can be seen without staining in visible light

C

Ethidium bromide stained DNA can be seen in visible light

D

Ethidium bromide stained DNA can be seen under exposure to UV light

Text Solution

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The correct Answer is:
D
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag in molecular biology for technique such as agarose gel electrophoresis. When exposed to ultraviolet light it will fluoresce with an orange colour intensifying almost 20 fold after binding to DNA
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