The role of DNA ligase in the construction of a recmobinant DNA molecule is

Options

A. Formation of phosphodiester bond between two DNA fragments
B. Formation of hydrogen bonds between sticky ends of DNA fragments
C. Ligation of all purine and pyrimidine bases
D. None of the above

Answer

A

Explanation

The mechanism of DNA ligase is to form two covalent phosphodiester bonds between 3’ hydroxyl ends of one nucleotide with the 5’ phosphate end of another