Significance of 'heat shock' method in bacterial transformation is to facilitate

Options

A. Binding of DNA to the cell wall
B. Uptake of DNA through membrane transport proteins
C. Uptake of DNA through transient pores in the bacterial cell wall
D. Expression of antibiotic resistance gene

Answer

C

Explanation

Heat shock transformation uses a calcium rich environment provided by calcium carbonate to counteract the electrostatic repulsion between the plasmid DNA and bacterial cellular membrane