Which of the following should be chosen for best yield if one were to produce a recmobinant protein in large amounts ?

Options

A. Laboratory flask of largest capacity
B. A stirred-tank bioreactor without in-lets and out-lest
C. A continuous culture system
D. Any of the above

Answer

C

Explanation

After having cloned the gene of interest and having optimized the conditions to induce the expression of the target protein one has to consider producing it on a large scale. Small volume cultures cannot yield appreciable quantities of product. To produce in large quantities the development of bioreactors where large volume of culture can be continuously processed is required.