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In rDNA technology in order to make the ...

In rDNA technology in order to make the bacterial host cells 'component' to accept the rDNA, these are kept in

A

Dilute solution of CsCl

B

Divalent anions such as phosphates

C

Chilled ethanol

D

Divalent cations such as calcium

Text Solution

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The correct Answer is:
D
To make the bacterial host cell ‘component’ it is treated with a specific concentration of a divalent cation such as calcium to increase pore size in cell wall. The cells are then incubated with recombinant DNA on ice followed by placing them briefly at 42ºC and then putting it back on ice. This is called heat shock treatment. This enables the bacteria to take up the DNA
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