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Recombinant DNA is force to enter the ho...

Recombinant DNA is force to enter the host cells by incubating the cells with rDNA first

A

On ice followed by heat shock and then again on ice

B

At low temperature followed by heat shock and then on ice

C

In ethidium bromide followed by calcium salts

D

Into chilled ethanol followed by ice and then into CsCl

Text Solution

Verified by Experts

The correct Answer is:
A
The cells treated by chemical such as calcium so as to increase pore size in cell wall. The cells are incubated first with recombinant DNA on ice, followed by heat shock treatment at 42ºC and then putting it back on ice. This enables the bacteria to take up the recombinant DNA
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