The following cycle refer to the PCR prcess, name the factors or steps indicated with numbers

Options

A. (i) Taq polymerase (ii) Extension (iii) Ligation
B. (i) Primer (ii) Denaturation at 94ºC (iii) Taq polymerase
C. (i) Denaturation at 94ºC (ii) Taq polymerase (iii) Primer
D. (i) Taq polymerase (ii) Denaturation at `94^(@)` (iii) Primer

Answer

D

Explanation

Steps involved in PCR are :
Denaturation – It helps to denatured double strands of DNA at a very high temp about 90 – `92^@`C.
Annealing : After denaturation the 8 – 10 nucleotide long called primer annealed at 3’ – end which is then extend by thermostable enzyme Taq polymerase at `72^@` C obtained from Thermus aquaticus.
Extension : Extension of primer by enzyme Taq polymerase that is thermostable obtained from Thermus aquaticus