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Following enzymes /techniques are used i...

Following enzymes /techniques are used in the process of recombinant DNA technology
A. Eco RI to cut the isolated genome
B. DNA ligase
C. Protease and ribonuclease for removal of proteins and RNA from DNA
D. Production of recombination hosts
E. lysozyme for isolation of the genetic material (DNA)
F. gel electrophoresis for separation and isolation of DNA fragments
Mark the correct sequence of their use.

A

C, E, B, F, A, D

B

E, C, A, B, F, D

C

E, C, A, F, B, D

D

A, E, C, B, D, F

Text Solution

Verified by Experts

The correct Answer is:
C
Isolation of DNA by cleaving bacterial cell walls by Lysozyme. Then removal of proteins and RNA from DNA by protease and ribonuclease. Next Isolated DNA cleave at specific site by using restriction enzymes and DNA fragments are separated by agarose gel electrophoresis. Next DNA fragment of desired gene ligated by using a suitable vector and production of Recombinant host
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