An analysis of chromosomal DNA using the southern hybridization technique does not use

Options

A. Autoradiography
B. PCR
C. Electrophoresis
D. Blotting

Answer

B

Explanation

PCR is not included in this technique. Steps involved in southern blotting are : DNA digestion at specific site by using restriction endonuclease. Cleave DNA at specific site by using restriction endonuclease. Blotting of separate DNA fragment on nylon and nitrocellulose membrane. Known DNA sequences called probe are radio labelled. Hybridization and washing. Detection by autoradiography.