Home
Class 12
BIOLOGY
A mixture containing DNA fragments a, b,...

A mixture containing DNA fragments a, b, c and d with molecular weigths of `a+b=c,a gt b` and `d gt c`, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would be

A

b,ac,d

B

a,bc,d

C

c,b,a,d

D

b,a,d,c

Text Solution

Verified by Experts

The correct Answer is:
A
Electrophoresis is a technique that uses differences in electrical charges to separate the molecule in a mixture. Since DNA molecules have negative charges they migrate towards the positive pole when placed in an electrical field. This rate of migration depends on shape and the charge to mass ratio. The smaller the DNA molecule faster is the migration. So on the basis of this “d” is the one with higher molecular weight and “b” is with lowest molecular weight. The order is b ,a ,c ,d.
Promotional Banner

Similar Questions

Explore conceptually related problems

a = e gt b = d gt c

In gel electrophoresis, negatively/positively charged DNA fragments placed over cathode will move towards anode of an electrical field.

If a, b, c, d are positive numbers in HP then prove that ad gt bc .

If a +b gt c+d and b + c gt a+d, then it is definite that

Read the following statements and find out the incorrect statements will reference to agarose gel electrophoresis (i) Now-a-days the most commonly used matric is agarose which is a synthetic polymer extracted from sea weeds (ii) Large the frament size, the farther it moves (iii) We can not see pure DNA in UV light without staining (iv) DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel