During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of POR will be affected first?

Options

A. Extension
B. Denaturation
C. Ligation
D. Annealing

Answer

B

Explanation

• High temperature (about `94^@` C) is required for the process of denaturation which is the first step of PCR. If this is not maintained, the denaturation of DNA is not done.
• Annealing is performed at `50^@-60^@ C` which is the second step that can get affected.
• Addition of nucleotides to the primer, synthesizing a new DNA strand using only the template sequences with the help of enzyme DNA polymerase is called primer extension/ polymerisation.
• Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase.