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Assertion : Recognition site should be p...

Assertion : Recognition site should be perfectly single and responsive to commonly used restriction enzymes.
Reason: In pNR 322 Alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

A

If both the assertion and the reason are true and the reason is a correct explanation of the assertion

B

If both the assertion and reason are true but the reason is not a correct explanation of the assertion

C

If the assertion is true but the reason is false

D

If both the assertion and reason are false

Text Solution

Verified by Experts

The correct Answer is:
B
The vector requires very few or single recognition size for the restriction enzymes. The presence of more than one recognition sites within the vector will generate several fragments leading to complication in gene cloning.
Ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. Example is legating a foreign DNA at the BamHI site of tetracycline resistance gene in the vector pBR322. The recombinant Plasmid will lose tetracycline resistance due to insertion of foreign DNA.
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