Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(i) synthesis of gene (DNA) for human insulin artificially (ii) culturing transformed E.coli in bioreactors. (iii) purification of humulin. (iv) insertion of human insulin gene into plasmid. (v) introduction of recombinant plasmid into E.coli. (vi) extraction of recombinant gene product from E.coli

Options

A. (ii), (i), (iv), (iii), (v), (vi)
B. (i), (iii), (v), (vi), (ii), (iv
C. (i), (iv), (v), (ii), (vi), (iii)
D. (iii), (v), (ii), (i), (vi), (iv)

Answer

C

Explanation

The steps in the production of genetically engineered insulin are :
* An insulin producing gene is isolated from human insulin extracted from pancreas cells.
* A plasmid DNA is extracted from E. coli and converted into a plasmid vector by digesting it with restriction enzyme.
* The human insulin producing gene is then inserted into plasmid vector forming recombinant DNA.
* Later on this recombinant DNA is introduced into the bacterium now called as recombinant bacterium.
* This recombinant bacterium multiplies in a fermentation tank and produces a large number of copies of human insulin.
* Finally insulin is extracted, purified and packed.