Which is not correctly matched

Options

A. Lipase - Hydrolysis of fats
B. Isomerases - Joining of similar substrate and management of substrate
C. Polymerase - Chain elongation
D. DNA ligase - Breaks DNA strand into two segments

Explanation

To determine which option is not correctly matched, we will analyze each option provided in the question.

### Step-by-Step Solution:

1. **Option 1: Lipase - Hydrolysis of Fats**
- Lipase is an enzyme that catalyzes the hydrolysis of fats (triglycerides) into glycerol and fatty acids. This statement is correct.
- **Conclusion**: Correctly matched.

2. **Option 2: Isomerase - Joining of Similar Substrates**
- Isomerase is an enzyme that catalyzes the conversion of a molecule into its isomers. It does not join substrates but rather rearranges them. However, it can be involved in reactions that may seem like joining but fundamentally it changes the structure without adding new substrates.
- **Conclusion**: This is somewhat misleading, but it can be considered as correctly matched in the context of rearranging similar substrates.

3. **Option 3: Polymerase - Chain Elongation**
- Polymerase is an enzyme that synthesizes long chains or polymers by adding nucleotides to a growing DNA or RNA strand. This is indeed correct.
- **Conclusion**: Correctly matched.

4. **Option 4: DNA Ligase - Breaking of DNA Strand into Two Segments**
- DNA ligase is an enzyme that joins two DNA strands together, particularly during DNA replication and repair. It does not break DNA strands; that function is performed by enzymes like DNA helicase. Therefore, this statement is incorrect.
- **Conclusion**: Not correctly matched.

### Final Answer:
The option that is not correctly matched is **Option 4: DNA Ligase - Breaking of DNA Strand into Two Segments**.

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